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KMID : 1098420160240040317
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Korean Journal of Medicinal Crop Science
2016 Volume.24 No. 4 p.317 ~ p.322
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Development of Chloroplast DNA-Based Simple Sequence Repeat Markers for Angelica Species Differentiation
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Park Sang-Ik
Kim Se-Rim
Gil Jin-Su
Lee Yi
Kim Ho-Bang
Lee Jung-Ho
Kim Seong-Cheol
Jung Chan-Sik
Um Yu-Rry
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Abstract
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Background: In the herbal medicine market, Angelica gigas, Angelica sinensis, and Angelica acutiloba are all called "Danggui" and used confusingly. We aimed to assess the genetic diversity and relationships among 14 Angelica species collected from different global seed companies. Toward this aim we developed DNA markers to differentiate the Angelica species.
Methods and Results: A total of 14 Angelica species, A. gigas, A. acutiloba, A. sinensis, A. pachycarpa, A. hendersonii, A. arguta, A. keiskei, A. atropurpurea, A. dahurica, A. genuflexa, A. tenuissima, A. archangelica, A. taiwaniana, and A. hispanica were collected. The genetic diversity of all 14 species was analyzed by using five chloroplast DNA-based simple sequence repeat (SSR) markers and employing the DNA fragment analysis method. Each primer amplified 3 - 12 bands, with an average of 6.6 bands. Based on the genetic diversity analysis, these species were classified into specific species groups. The cluster dendrogram showed that the similarity coefficients ranged from 0.77 to 1.00.
Conclusions: These findings could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Angelica species. The analysis of polymorphic SSRs could provide an important experimental tool for examining a range of issues in plant genetics.
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KEYWORD
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Angelica, Chloroplast DNA, Simple Sequence Repeat
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